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This exciting new class of polymer-based fluorescent molecules will change the future of flow cytometry, by bringing new power and utility to your violet (405) laser.
These polymers possess both a high energy absorption capacity, and high emission efficiency (quantum yield). When conjugated to antibodies, they bring dramatic improvements in brightness and signal-to-noise ratio on labeled cells, over the current choices for the violet laser.
Improvements over current violet-excited dyes
...that result in
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In contrast to traditional dyes, the Brilliant Violent fluorescent polymers are about 80 kD in size, consisting of fluorescent monomeric subunits that act cooperatively along the entire length of the polymer backbone. The energy is conducted much like a molecular antennae to capture light and pass it down the antennae like a lightning rod. This creates an extinction coefficient that is substantially larger than standard organic fluorescence, and when conjugated to antibodies, this results in high intensity brightness on labeled cells.
Brilliant Violet Technology
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These conjugated polymers are comprised of alternating single and double bonds and aromatic units. This repeating bond structure creates a continuous π-orbital system and extended electronic delocalization. A configuration that confers several unique and tunable optical properties, (including large extinction coefficients (>106 M-1cm-1), intense photoluminescence, and massive collective response); that collectively address fundamental limitations in fluorescence detection sensitivity.
Current violet excited dyes used in flow cytometry, like Pacific Blue™ and BD Horizon V450, have high quantum yields but limited extinction coefficients. Because the extinction coefficient of a conjugated polymer is directly proportional to the degree of polymerization (or number of repeat units), these structures are designed for improved brightness. Further, as these polymers are derived from common synthetic organic and polymer chemistry techniques is it possible to manufacture reagents very reproducibly; in terms of size, conjugation sites, and optical properties.
In addition, unlike quantum dots, these new conjugated polymers have discrete excitation spectra, similar to that of organic dyes, which minimizes potential issues with cross-beam compensation.
Brilliant Violet 421™
In particular, Brilliant Violet 421™ antibodies consistently stain at brighter levels than PE, the brightest known fluorochrome, bringing unparalleled sensitivity and resolution to the violet laser.
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BV421™ has both a narrow excitation curve and a narrow emission curve, which means less compensation than other fluorochromes.
BV421™ enables resolution of antigens with directly conjugated antibodies, providing increased sensitivity for resolving weakly expressing antigens and expanding options for multi-color panels.
Fluorochrome Parameter Comparison
Staining Index (S.I.) values are derived from RBC-lysed, human whole blood cells stained at optimal concentration for each conjugate and analyzed on an iCyt Synergy™. (S.I. = mean positive - mean background / 2 x S.D. background)
Anti-Human CD3 - BV421
Anti-Human CD56 - BV421
Anti-Mouse CD4 - BV421
New! Brilliant Violet™ 570 iCyt is proud to announce the latest addition to our Brilliant Violet family of products; the BV570™ portfolio.
BV570 is a tandem fluorophore of the BV421 polymer conjugated to Cy3 (BV570/Cy3).
Like our BV421 products, these conjugates are excited by your 405 laser, but emit in the 570 range further expanding your multicolor options.
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 RBC-lysed human whole blood cells stained with iCyt anti-human CD3 (UCHT1) conjugated to Brilliant Violet 570™, and run on the iCyt Eclipse™ flow cytometer. |
 RBC-lysed human whole blood cells stained with iCyt anti-human CD127() conjugated to Brilliant Violet ™ 570 and CD25 () conjugated to BV421™, and run on the iCyt Eclipse™ flow cytometer, gated on lymphocytes. |
Brilliant Violet™ conjugates offer:
| • | Consistent bright staining | |
| • | Simple, ready-to-use, low volume (5uL) test size | |
| • | Compatibility with standard staining buffers | |
| • | Fixation stability | |
| • | Extensive antibody selection | |
| • | iCyt/Sony Satisfaction Guarantee | |
You simply have to try these new 405-excited conjugates!
Brilliant Violet antibodies consistently bring unparalleled sensitivity and resolution to the violet laser.
Brilliant Violet™ antibody conjugates can enable you to generate clearer, more accurate data than ever before.
Copyright ©2011 iCyt Mission Technology. All rights reserved. The iCyt name, logo, Synergy and Eclipse are trademarks of iCyt Mission Technology. Brilliant Violet and Powered by Sirigen are trademarks of Sirigen, Ltd. Pacific Blue is a trademarks of and licensed under patents assigned to Life Technologies Corporation. All other trademarks are property of their respective owners. |
